Mycobactin, a growth factor for Mycobacterium johnei. 1. Isolation from Mycobacterium phlei

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Isolation and structure of mycobactin T, a growth factor from Mycobacterium tuberculosis.

1. A growth factor for Mycobacterium johnei has been isolated from Mycobacterium tuberculosis as an iron complex; it has been named ferric mycobactin T. 2. Ferric mycobactin T as isolated is a mixture of at least four closely related components differing only in their fatty acid side chains. 3. Mycobactin T resembles mycobactin P in structure but differs in the following respects: (a) the aroma...

The Structure of Mycobactin P, a Growth Factor for Mycobacterium Johnei, and the Significance of Its Iron Complex.

1. The growth factor from Mycobacterium phlei is now named mycobactin P to distinguish it from related but chemically distinct growth factors from other species of mycobacteria. It is shown to comprise four closely similar chemical entities differing only in the fatty side chain. 2. The structure of the main component (85%) of mycobactin P has been established and the absolute configuration of ...

The growth of Mycobacterium tuberculosis and Mycobacterium johnei in a modification of Dubos medium.

The success achieved in growing Mycobacterium tuberculosis in liquid medium (Dubos & Davis, 1946) has aroused interest in the possibility of growing M . johnei in this fluid or similar media, modified to contain the essential growth-promoting substances for these organisms. So far no success has been achieved (Davis & Dubos, 1948; Taylor, 1950). The combination of Tween 80 and bovine albumin fr...

Genetic transfer in Mycobacterium phlei.